In the diff laser channel, red blood cells and platelets can be lysed and eosinophils specifically stained, allowing complete distinction of lymphocytes, monocytes, eosinophils from basophils and neutrophils as detected by laser light scattering and flow cytometry; Abnormal lymphocytes and giant immature cells were simultaneously screened and are shown on diff scatter plots; Also, floating cluster classification was used, and the scatter plot was automatically adjusted to accommodate the inter sample variation